Wade Van Horn

ISTB1 481E
ISTB1
TEMPE
Assistant Professor
Faculty
TEMPE Campus
Mailcode
6401

Biography

Wade Van Horn is an assistant professor in the School of Molecular Sciences and is an investigator with the Biodesign Institute's Center for Personalized Diagnostics, and the Magnetic Resonance Research Center. He joined Arizona State University in 2012 after an American Heart Association postdoctoral fellowship at Vanderbilt University School of Medicine in the Department of Biochemistry and the Center for Structural Biology. He received his Ph.D. from the University of Utah's Department of Chemistry. His current interests focus on the interplay between biomolecular function and structure, especially as it relates to human physiology and pathophysiology.

Education

Ph.D. Chemistry, University of Utah 2007

Research Interests

Our research interests focus on the roles of membrane proteins in human health and disease, with a particular interest in membrane proteins with therapeutic potential. Membrane proteins, as a class, make up the majority of therapeutic targets and play essential roles in biology and pathophysiology. We investigate the structure, dynamics, and function of this class of proteins with an interdisciplinary approach synthesizing the output from multiple disparate techniques which allows us to tackle challenging yet biomedically relevant problems. Our investigations focus on the following three themes:

1) TRP channel gating and modulation. TRPM8 functions as the primary cold sensor in humans where it integrates, thermo-, chemical-, and voltage-dependencies and is modulated by other proteins and lipids. This channel has significant therapeutic potential in diverse and important diseases such as cancer, chronic pain, obesity, and diabetes. We seek to understand the mechanism of how TRPM8 integrates distinct stimuli and modulators in an effort to unlock the therapeutic potential of TRPM8.

2) Membrane protein structural enzymology. Our lab focuses on two membrane enzymes; vitamin K epoxide reductase (VKOR) and undecaprenol kinase (UDPK). VKOR is the target of the popular anticoagulant warfarin (also Coumadin�) and is one of the hallmark targets of personalized medicine. Undecaprenol kinase is a known virulence factor in gram-positive bacteria and an excellent target for narrow spectrum antibiotic development. For these enzymes we seek to understand the relationship between structure, function, and dynamics.

3) Methods Development for membrane protein structural biology. In an effort to push the boundaries of membrane protein structural biology, we are exploring the use of new, and fine-tuning existing membrane mimics that are compatible with solution NMR. Of particular interest is optimizing reverse micelles as hosts for membrane proteins which have the particular advantage of significantly increasing the size and complexity of NMR-accessible membrane targets.

To accomplish these goals, our research program relies on a number of interdisciplinary techniques including nuclear magnetic resonance spectroscopy (NMR), electrophysiology, enzymology, and computational structural biology.

Publications

“New Pieces to an Old Puzzle: Identifying the Warfarin-binding Site that Prevents Clotting,” Hilton JK, Van Horn WD, Nature Structure Molecular Biology 24 5-6 (2017)

“A Parallel Stranded G-quadruplex Composed of Threose Nucleic Acid (TNA),” Liao JY, Anosova I, Bala S, Van Horn WD, Chaput JC, Biopolymers 10 e22999 (2017)

“The structural diversity of artificial genetic polymers,” Anosova I, Kowal, EA, Dunn MR, Chaput JC, Van Horn WD, Egli M, Nucleic Acids Res 44 1007-1021 (2016)

“Structural Basis for KCNE3 Modulation of Potassium Recycling in Epithelia,” Kronce BM, Van Horn WD*, Smith J, Kang C, Welch RC, Song Y, Nannemann DP, Sisco NJ, George AL, Meiler J, Vanoye CG, Sanders CR, Science Advances 2 e1501228 (2016)

“Structural Insights into Conformational Differences between DNA/TNA and RNA/TNA Chimeric Duplexes,” Anosova I, Kowal EA, Sisco NJ, Sau S, Liao J, Bala S, Rozners E, Egli M, Chaput JC, Van Horn WD, ChemBioChem 17 1705-1708 (2016)

“Implications of Human Transient Receptor Potential Melastatin 8 (TRPM8) Channel Gating from Menthol Binding Studies of the Sensing Domain,” Rath P, Hilton JK, Sisco NH, Van Horn WD, Biochemistry 55 114-124 (2016)

“Understanding Thermosensitive Transient Receptor Potential Channels as Versatile Polymodal Cellular Sensors,” Hilton JK, Rath P, Helsell CV, Beckstein O, Van Horn WD, Biochemistry 54 2401-2413 (2015)

“NMR Structure of Francisella tularensis Virulence Determinant Reveals Structural Homology to Bet v1 Allergen Proteins,” Zook, J., Mo, G, Sisco, NJ, Craciunescu, FM, Hansen, DT, Baravati, B, Cherry, B, Sykes, K, Wachter, R, Van Horn, WD, Fromme, P., Structure 23 1116-1122 (2015)

"Bicelles at low concentrations," Z. Lu, W.D. Van Horn, J. Chen, S. Mathew, R. Zent, C.R. Sanders, Mol. Pharm. 336 752-761 (2012)

"The amyloid precursor protein has a flexible transmembrane domain and binds cholesterol," P.J. Barrett, Y. Song, W.D. Van Horn, E.J. Hustedt, J.M Schafer, A. Hadziselimovic, A.J. Beel, C.R. Sanders, Science 336 1168-1171 (2012)

"Prokaryotic Diacylglycerol Kinase and Undecaprenol Kinase," W.D. Van Horn, C.R. Sanders, Annu. Rev. Biophys. 41 61-81 (2012)

"Working model for the structural basis for KCNE1 modulation of the KCNQ1 potassium channel," W.D. Van Horn, C.G. Vanoye, C.R. Sanders,Curr. Opin. Struct. Biol. 21 283-291 (2011)

"Functional Delivery of a Membrane Protein into Oocyte Membranes Using Bicelles," C. Kang, C.G. Vanoye, R.C. Welch, W.D. Van Horn, C.R. Sanders, Biochemistry 49 653-655 (2010)

"The Impact of Window Functions on NMR-Based Paramagnetic Relaxation Enhancement Measurements in Membrane Proteins," W.D. Van Horn, A.J. Beel, C. Kang, C.R. Sanders, Biochim. Biophys. Acta 1798 140-149 (2010)

"Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins," H. Kim, S.C. Howell, W.D. Van Horn, Y.H. Jeon, C.R. Sanders, Prog. Nucl. Magn. Reson. Spectrosc. 55 335-360 (2009)

"Reverse Micelle Encapsulation as a Model for Intracellular Crowding," W.D. Van Horn, M.E. Ogilvie, P.F. Flynn, J. Am. Chem. Soc. 131 8030-8039(2009)

"NMR Solution Structure of the Membrane-Integral Diacylglycerol Kinase," W.D. Van Horn, H. Kim, C.D. Ellis, A. Hadziselimovic, E.S. Sulistijo, M.D. Karra, C. Tian, F.D. Sönnichsen, C.R. Sanders, Science 324 1726-1729 (2009)

"Use of reverse micelles in membrane protein structural biology," W.D. Van Horn, M.E. Ogilvie, P.F. Flynn, J. Biomol. NMR 40 203-211 (2008)

"NMR Studies of Encapsulated Macromolecules," P.F. Flynn, A.K. Simorellis, W.D. Van Horn, Annual Reports in NMR Spectroscopy 62 179-219(2007)

"Dynamics of Low Temperature Induced Water Shedding from AOT Reverse Micelles," A.K. Simorellis, W.D. Van Horn, P.F. Flynn, J. Am. Chem. Soc. 128 5080-5090 (2006)

"Low Temperature Studies of Encapsulated Proteins," W.D. Van Horn, A.K. Simorellis, P.F. Flynn, J. Am. Chem. Soc. 127 13553-13560 (2005)

Research Activity

Courses

Spring 2018
Course NumberCourse Title
BCH 463Biophysical Chemistry
MBB 493Honors Thesis
Fall 2017
Course NumberCourse Title
MBB 492Honors Directed Study
MBB 495Undergraduate Research
BCH 561Adv Topics in Biochemistry
Spring 2017
Course NumberCourse Title
BCH 463Biophysical Chemistry
MBB 493Honors Thesis
BCH 561Adv Topics in Biochemistry
Fall 2016
Course NumberCourse Title
MBB 492Honors Directed Study
MBB 495Undergraduate Research
Spring 2016
Course NumberCourse Title
BCH 501Current Topics in Biochemistry
CHM 598Special Topics
BCH 598Special Topics
Fall 2015
Course NumberCourse Title
BCH 463Biophysical Chemistry
BCH 598Special Topics
Spring 2015
Course NumberCourse Title
BCH 501Current Topics in Biochemistry
CHM 598Special Topics
BCH 598Special Topics
Fall 2014
Course NumberCourse Title
BCH 463Biophysical Chemistry
BCH 598Special Topics
Spring 2014
Course NumberCourse Title
BCH 463Biophysical Chemistry
CHM 598Special Topics
BCH 598Special Topics
Fall 2013
Course NumberCourse Title
BCH 501Current Topics in Biochemistry
BCH 598Special Topics