Po-Lin Chiu

1001 S McAllister Avenue
Biodesign A230A
Assistant Professor
Faculty
TEMPE Campus
Mailcode
1604

Biography

Po-Lin Chiu has always been interested in understanding how biological form follows its function. He earned his bachelor's in Pharmacy at National Taiwan University, Taipei, and in addition to the clinical practice, he started to work in the laboratory and study a viral helicase using X-ray crystallography. To expand knowledge, he pursued his master's degree in electrical engineering at National Taiwan University, Taipei, and has the expertise in optics and signal processing. His Ph.D. was then completed in biophysics at the University of California, Davis, focusing on electron microscopy of a bacterial cyclic nucleotide-modulated channel. After graduation, he moved to Harvard Medical School, Boston, as a postdoctoral fellow to study how lipids interact with membrane proteins using cryogenic electron microscopy (cryo-EM). Now at ASU he continues his research investigations on lipid-protein interaction with a focus on the receptor complex of axonal regeneration using the cryo-EM method combined with biophysical and biochemical techniques. 

Education

  • Ph.D. Biophysics, University of California-Davis 2009
  • M.EE. Electrical Engineering, National Taiwan University, Taipei, Taiwan 2001
  • B.Sc. Pharmacy, National Taiwan University, Taipei, Taiwan 1999

Google Scholar

Research Interests

Our laboratory is interested in the membrane protein structures and lipid-protein interaction.  The current focus is to investigate the interaction between each component of the protein complexes, in the context of the membrane bilayer, that inhibit axonal regeneration.  We are also interested in method development in single-particle cryo-electron microscopy (cryo-EM).  

Publications

Aryal, R.P.*, Kwak, P.B.*, Tamayo, A.G., Chiu, P.-L., Walz, T., and Weitz, C.J. 2017. Macromolecular assemblies of the mammlian circadian clock. Mol Cell 67:770-782. (*equal contribution). 

Kalbermatter, D.*, Chiu, P.-L.*, Jeckelmann, J.-M., Ucurum, Z., Walz, T., and Fotiadis, D. 2017. Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change. J Struct Biol 199:39-45(*equal contribution)

Leksa, N.C., Chiu, P.-L., Bou-Assaf, G.M., Quan, C., Liu, Z., Goodman, A.B., Chambers, M.G., Tsutakawa, S.E., Hammal, M., Peters, R.T., Walz, T., and Kulman, J.D. 2017. The structural basis for the functional comparability of factor VIII and the long-acting variant rFVIIIFc. J Thromb Haemost 15:1167-1179. 

Klara, S.S., Saboe, P.O., Sines, I.T., Babaei, M., Chiu, P.-L., DeZorzi, R., Dayal, K., Walz, T., Kumar, M., and Mauter, M.S. 2016. Magnetically directed two-dimensional crystallization of OmpF membrane proteins in block copolymers. J Am Chem Soc 138:28-31.

Chiu, P.-L.*, Li, X.*, Li, Z., Beckett, B., Brilot, A.F., Grigorieff, N., Agard, D.A., Cheng, Y., and Walz, T. 2015. Evaluation of super-resolution performance of the K2 electron counting camera using 2D crystals of aquaporin-0. J Struct Biol 192:163-173. (*equal contributions)

Kalbermatter, D., Jeckelmann, J.-M., Chiu, P.-L., Ucurum, Z., Walz, T., and Fotiadis, D. 2015. 2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli. J Struct Biol 191:376-380.

Chiu, P.-L.*, Bou-Assaf, G.*, Chhabra, E.S., Chambers, M.G., Liu, L., Peters, R., Kulman, J.D., and Walz, T. 2015. Mapping the interaction between factor VIII and von Willebrand factor by electron microscopy and mass spectrometry. Blood 126:935-938. (*equal contributions)

Hite, R.K.*, Chiu, P.-L.*, Schuller, J., and Walz, T. 2015. Effect of lipid head groups on double-layered two-dimensional crystals formed by aquaporin-0. PLoS ONE 10:e0117371. (*equal contributions)

Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P.-L., Rangl, M., Scheuring, S., Nimigean, C.M., and Stahlberg, H. 2014. Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nat Commun 5:3106-3115.

Hopkins, L.E., Patchin, E.S., Chiu, P.-L., Brandenberger, C., Smiley-Jewell, S., and Pinkerton, K.E. 2014. Nose-to-brain transport of aerosolized quantum dots following acute exposure. Nanotoxicol 8:885-893.

Evans, J.E., Jungjohann, K.L., Wong, P.C.K., Chiu, P.-L., Dutrow, G.H., Arslan, I., and Browning, N.D. 2013. Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. Micron 43:1085-1090.

Chiu, P.-L., Kelly, D.F., and Walz, T. 2011. The use of trehalose in the preparation of specimens for electron microscopy. Micron 42:762-772.

Paoli, E.-E., Kruse, D.E., Seo, J.W., Zhang, H., Kheirolomoom, A., Watson, K.D., Chiu, P.-L., Stahlberg, H., and Ferrara, K.W. 2010. An optical and microPET assessment of thermally-sensitive liposome biodistribution in the Met-1 tumor model: importance of formulation. J Control Release 143:13-22.

Chiu, P.-L., Pagel, M.D., Evans, J.E., Chou, H.-T., Zeng, X., Gipson, B., Stahlberg, H., and Nimigean, C.M. 2007. The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 Å resolution. Structure 15:1053-1064.

Renault, L., Chou, H.-T., Chiu, P.-L., Hill, R.M., Zeng, X., Gipson, B., Zhang, Z.Y., Cheng, A., Unger, V., and Stahlberg, H. 2006. Milestones in electron crystallography. J Comput Aided Mol Des 20:519-527.

Chiu, P.-L., Wu, C.-Y., Taso, J.-H., and Chang, F.-H. 2001. Drug delivery through liposome by ultrasonic cavitation. Biomed Eng-App, Basis, & Comm 13:47-52.   

Research Activity

Assistant Professor, 2016-now.
School of Molecular Sciences and The Biodesign Institute, Arizona State University, Tempe, Arizona.

Postdoctoral Fellow, 2010-2016.
Department of Cell Biology, Harvard Medial School, Boston, Massachusetts.
Laboratory of Thomas Walz.

Postdoctoral Researcher, 2009-2010.
Section of Molecular and Cellular Biology, University of California, Davis, California.
Laboratory of Henning Stahlberg.
 

 

Courses

Spring 2018
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Fall 2017
Course NumberCourse Title
BCH 598Special Topics
Fall 2016
Course NumberCourse Title
BCH 598Special Topics