Po-Lin Chiu

Peer-reviewed papers

32. Waheeda, K., Kitchel, H., Wang, Q., and Chiu, P.-L. Molecular mechanism of Rubisco activase: dynamic assembly and Rubisco remodeling. Front Mole Biosci 10 (2023). 

31. Puskar, R., Truong, C.D., Swain, K., Chowdhury, S., Chan, K.-Y., Li, S., Cheng, K.-W., Wang, T.Y., Poh, Y.-P., Mazor, Y., Liu, H., Chou, T.-F., Nannenga, B.L., and Chiu, P.-L. Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria. Nature Commun 13, 5824 (2022). 

30. Narayanan, R.P., Procyk, J., Nandi, P., Prasad, A., Xu, Y., Poppleton, W., Williams, D.R., Zhang, F., Yan, H., #Chiu, P.-L., #Stephanopoulos, N., and #Šulc, P. Coarse-grained simulations for the characterization and optimization of hybrid protein-DNA nanostructures. ACS Nano 16, 14086-14096 (2022). 

29. DeVore, K. and Chiu, P.-L. Probing structural perturbation of biomolecules by extracting cryo-EM data heterogeneity. Biomolecules 12, 628 (2022). 

28. Baker, A.T., Boyd, R.J., Sarkar, D., Crespo, A.T., Chan, C.K., Bates, E., Waraich, K., Vant, J., Wilson, E., Truong, C.D., Lipka-Lloyd, M., Fromme, P., Vermaas, J., Williams, D., Machiesky, L., Heurich, M., Nagalo, B.M., Coughlan, L., Umlauf, S., Chiu, P.-L., Rizkallah, P.J., Cohen, T.S., Parker, A.L., Singharoy, A., and Borad, M.J. ChAdOx1 interacts with CAR and PF4 with implications for thrombosis with thrombocytopenia syndrome. Sci Adv 7 (2021). (doi: 10.1126/sciadv.abl8213) 

27. Truong, C.D., Williams, D., Zhu, M., Wang, J.C.-H. and Chiu, P.-L. Sample preparation using lipid monolayer for electron crystallographic studies. J Vis Exp 177, e63015 (2021). 

26. Zhang, X., Gui, L., Li, S., Nandi, P., Columbres, R.C., Wong, D.E., Moen, D.R., Lin, H.J., #Chiu, P.-L., and #Chou, T.-F. Conserved L464 in p97 D1-D2 linker is critical for p97 cofactor regulated ATPase activity. Biochem J 478, 3185-3204 (2021). (doi: 10.1042/BCJ20210288) (#equal contributions) 

25. Nandi, P., Li, S., Columbres, R.C.A., Wang, F., Williams, D.R., Poh, Y.-P., #Chou, T.-F., and #Chiu, P.-L. Structural and functional analysis of disease-linked p97 ATPase mutant complexes. Int J Mole Sci 22, 8079 (2021). (doi: 10.3390/ijms22158079) (#equal contributions) 

24. Truong, C.D., Craig, T.A., Cui, G., Botuyan, M.V., Serkasevich, R.A., Chan, K.-Y., #Mer, G., #Chiu, P.-L., and #Kumar, R. Cryo-EM reveals conformational flexibility in apo DNA polymerase zeta. J Biol Chem 297, 100912 (2021). (doi: 10.1016/j.jbc.2021.100912) (#equal contributions) 

23. March, K., Venkatraman, K., Truong, C.D., Williams, D., Chiu, P.-L., and Rez, P. Protein secondary structure signatures from energy loss spectra recorded in the electron microscopy. J Microsc 282, 215-223 (2021). 

22. Godeshala, S., Miryala, B., Dutta, S., Christensen, M.D., Nandi, P., Chiu, P.-L., and Rege, K. A library of aminoglycoside-derived lipopolymer nanoparticles for delivery of small molecules and nucleic acids. J Materials Chem B 8, 8558-8572 (2020). 

21. Yang, J.-H., Williams, D., Kandiah, E., #Fromme, P., and #Chiu, P.-L. Structural basis of redox modulation on chloroplast ATP synthase. Commun Biol 3, 482 (2020). (#equal contributions) 

20. Chang, S.L.Y., Reineck, P., Williams, D., Bryant, G., Opletal, G., El-Demrdash, S.A., Chiu, P.-L., Õsawa, E., Barnard, A.S., and Dwyer, C. Dynamic self-assembly of detonation nanodiamond in water. Nanoscale 12, 5363-5367 (2020). 

19. Robertson, K.E., Craciunescu, F.M., Truong, C., Chiu, P.-L., Fromme, P., and Hansen, D.T. Membrane directed expression in Escherichia coli of virulence factors from the Lyme disease agent Borrelia burgdorferi. Sci Rep 9, 1-15 (2019). 

18. Steber, H.S., Gallante, C., Chiu, P.-L., and Mangone, M. The C. elegans 3’UTRome V2: an updated genomic resource to study 3’UTR biology. Genome Res 29, 2104-2116 (2019). 

17. Toporik, H., Li, J., Williams, D., Chiu, P.-L., and Mazor, Y. The structure of the photosystem I IsiA super-complex. Nat Struct Mole Biol 26, 443-449 (2019). 

16. Aryal, R.P., Kwak, P.B., Tamayo, A.G., Chiu, P.-L., Walz, T., and Weitz, C.J. Macromolecular assemblies of the mammalian circadian clock. Mol Cell 67, 770-782 (2017). 

15. *Kalbermatter, D., *Chiu, P.-L., Jeckelmann, J.-M., Ucurum, Z., Walz, T., and Fotiadis, D. Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change. J Struct Biol 199, 39-45 (2017). (*equal contributions)

14. Leksa, N.C., Chiu, P.-L., Bou-Assaf, G.M., Quan, C., Liu, Z., Goodman, A.B., Chambers, M.G., Tsutakawa, S.E., Hammel, M., Peters, R.T., Walz, T., and Kulman, J.D. The structural basis for the functional comparability of factor VIII and the long-acting variant rFVIIIFc. J Thromb Haemost 15, 1167-1179 (2017). 

13. Klara, S.S., Saboe, P.O., Sines, I.T., Babaei, M., Chiu, P.-L., DeZorzi, R., Dayal, K., Walz, T., Kumar, M., and Mauter, M.S. Magnetically directed two-dimensional crystallization of OmpF membrane proteins in block copolymers. J Am Chem Soc 138, 28-31 (2016). 

12. Chiu, P.-L., Li, X., Li, Z., Beckett, B., Brilot, A.F., Grigorieff, N., Agard, D.A., Cheng, Y., and Walz, T. Evaluation of super-resolution performance of the K2 electron counting camera using 2D crystals of aquaporin-0. J Struct Biol 192, 163-173 (2015). 

11. Kalbermatter, D., Jeckelmann, J.-M., Chiu, P.-L., Ucurum, Z., Walz, T., and Fotiadis, D. 2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli. J Struct Biol 191, 376-380 (2015). 

10. *Chiu, P.-L., *Bou-Assaf, G., Chhabra, E.S., Chambers, M.G., Liu, L., Peters, R., Kulman, J.D., and Walz, T. Mapping the interaction between factor VIII and von Willebrand factor by electron microscopy and mass spectrometry. Blood 126, 935-938 (2015). (*equal contributions)

9. *Hite, R.K., *Chiu, P.-L., Schuller, J., and Walz, T. Effect of lipid head groups on double-layered two-dimensional crystals formed by aquaporin-0. PLoS One 10, e0117371 (2015). (*equal contributions)

8. Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P.-L., Rangl, M., Scheuring, S., Nimigean, C.M., and Stahlberg, J. Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nat Commun 5, 3106-3115 (2014). 

7. Hopkins, L.E., Patchin, E.S., Chiu, P.-L., Brandenberger, C., Smiley-Jewell, S., and Pinkerton, K.E. Nose-to-brain transport of aerosolized quantum dots following acute exposure. Nanotoxicol 8, 885-893 (2014). 

6. Evans, J.E., Jungjohann, K.L., Wong, P.C.K., Chiu, P.-L., Dutrow, G.H., Arslan, I., and Browning, N.D. Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. Micron 43, 1085-1090 (2013). 

5. Chiu, P.-L., Kelly, D.F., and Walz, T. The use of trehalose in the preparation of specimens for electron microscopy. Micron 42, 762-772 (2011). 

4. Paoli, E.-E., Kruse, D.E., Seo, J.W., Zhang, H., Kheirolomoom, A., Watson, K.D., Chiu, P.-L., Stahlberg, H., and Ferrara, K.W. An optical and microPET assessment of thermally sensitive liposome biodistribution in the Met-1 tumor model: importance of formulation. J Control Release 143, 13-22 (2010). 

3. Chiu, P.-L., Pagel, M.D., Evans, J.E., Chou, H.-T., Zeng, X., Gipson, B., Stahlberg, H., and Nimigean, C.M. The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 Å resolution. Structure 15, 1053-1064 (2007). 

2. Renault, L., Chou, H.-T., Chiu, P.-L., Hill, R.M., Zeng, X., Gipson, B., Zhang, Z.Y., Cheng, A., Unger, V., and Stahlberg, H. Milestones in electron crystallography. J Comput Aided Mol Des 20, 519-527 (2006). 

1. Chiu, P.-L., Wu, C.-Y., Taso, J.-H., and Chang, F.-H. Drug delivery through liposome by ultrasonic cavitation. Biomed Eng-App Basis & Comm 13, 47-52 (2001). 

Conference papers

14. Chiu, P.-L. Structural basis for excited light transfer within a bacterial photosynthetic supercomplex. Biophys J (2023). 

13. Chan, K.-Y. Structural study of regulated intramembrane proteolysis of p75NTR by gamm-secretase. J Alzheimer Assoc 17, e053458 (2022). 

12. Chan, K.-Y., Poh, Y.-P., and Chiu, P.-L. Structural study of regulated intramembrane proteolysis of the p75 neurotrophin receptor by gamma-secretase. Biophys J (2022). 

11. Waheeda, K. and Chiu, P.-L. Complex formation of Rubisco and Rubisco activase. Biophys J (2022). 

10. Chiu, P.-L. Evaluation of hollow-cone electron imaging on biomolecules. Biophys J (2022). 

9. Orjuela, J.D., Chiu, P.-L., Walz, T., de Groot, B.L., and Aponte-Santamaria, C.A. The interplay between cholesterol and aquaporin-0. Biophys J (2022). 

8. Nandi, P., Li, S., Coulmbres, R.C.A., Wang, F., Williams, D.R., Poh, Y.-P., Chou, T.-F., and Chiu, P.-L. Cryo-EM reveals altered domain-domain communications in dementia-mutant R155H human p97/VCP ATPase. Biophys J (2022).  

7. Chiu, P.-L., Yang, J.-H., Williams, D., Kandiah, E., Fromme, P. Redox modulation on chloroplast ATP synthase. Acta Crystallographica Section A. Foundations and Advances (2021). 

6. Nandi, P., Li, S., Columbres, R.C.A., Wang, F., Williams, D.R., Malyutin, A.G., Poh, Y.-P., Chou, T.-F., and Chiu, P.-L. Structural and functional analysis of p47 cofactor binding on the p97 disease mutant. Microsc & Microana (2021). 

5. Nandi, P., Poh, Y.-P., and Chiu, P.-L. Structural studies of the neuronal apoptotic complex of proNGF-p75NTR-sortilin. Biophys J (2021). 

4. Yang, J.-H., Williams, D., Kandiah, E., Fromme, P., and Chiu, P.-L. Redox Modulation on Chloroplast ATP Synthase. Microsc & Microana (2020). doi: 10.1017/S1431927620013458. 

3. Nandi, P., and Chiu, P.-L. Structural studies using cryo-EM to unravel mechanistic details of p47 binding to p97. Biophys J 118, 501a (2020). doi: 10.1016/j.bpj.2019.11.2765. 

2. Luu, D., Chiu, P.-L., and Van Horn, W.D. Isolation of functional temperature activated transmembrane domain of human TRPM8. Biophys J (2020). 

1. Yang, J.-H., Fromme, P., and Chiu, P.-L. Mechanism of light regulation on chloroplast ATP synthase revealed by single-particle cryo-EM. BBA-Bioenergetics 1859, e84-e85 (2018).

Book chapters

1. Chan, K.-Y., Truong, C.D., Poh, Y.-P., and **Chiu, P.-L. Sample preparation and data collection for electron crystallographic studies on membrane protein structures and lipid-protein interaction. Methods in Mole Biol 2215, 247-265 (2021).